General Western Blot Reagents
LICOR WesternSure ECL Stripping Buffer for Chemiluminescent Westerns (100 mL)
WesternSure ECL Stripping Buffer is ideal for stripping and reprobing chemiluminescent Western blots. This Western blot stripping buffer. It removes primary and secondary antibodies from PVDF or nitrocellulose membranes, maintains target antigen integrity for efficient reprobing, and it does not require hazardous shipping, unlike many other stripping buffers. Stripping buffers are not recommended if bands need to be quantified. Note: WesternSure ECL Stripping Buffer is not recommended for loading amounts over 30 µg. It will demonstrate decreased performance when used at 2X or 5X concentration and heating the solution will not increase the stripping efficiency.
Abcam Bradford Reagent, 500ML
Bradford reagent is an improved formulation for protein quantification, offering higher tolerance to detergent contamination and reduced protein-to-protein variation compared to traditional bradford assays. - Cited in over 30 publications - Excellent linearity across a defined range of protein concentrations - Color Change – binds to protein, causing a shift in absorption from 465 nm to 595 nm with a color change from brown to blue
The product is subject to the following: Abcam Restricted Use Statement
LICOR GAR/GAM CellTag™ 520 Stain ICW Kit IV
GAR/GAM CellTag 520 Stain ICW Kits provide detection reagents for cell-based In-Cell Western™ Assays. This cost-effective normalization method makes quantification of the target protein more precise. CellTag 520 Stain accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number. This kit includes reagents to perform an In-Cell Western Assay and CellTag 520 Stain to normalize well-to-well variations in cell number. The Kit Components are IRDye 800CW Goat anti-Rabbit Secondary Antibody (0.5 mg), IRDye 680RD Goat anti-Mouse Secondary Antibody (0.5 mg), Intercept (TBS) Blocking Buffer (1 x 500 mL), CellTag 520 Stain (2 x 50 nmol), and Kit Protocol.
ProteinSimple 12-230 KDA WES SEPARATION MODU
12-230 kDa Wes Separation Module, 2 x 25 capillary cartridges.Use with a Detection Module. Reagents for 50 data points includes two parts: Separation box with 2x Capillary Cartridges (25 capillaries per cartridge), 2x Wes 12-230 kDa pre-filled Plates (pre-filled with Separation Matrix 2, Stacking Matrix 2, Split Running Buffer 2 and Matrix Removal Buffer), Sample Buffer, Wash Buffer (Store at RT), plus 1x Standard Pack 1 clamshell with 2x DTT and 2x FL master mix with 29 kDa System Control protein. (Store at 4 C).
Abcam Carbo x y-DCFDA N-succinimidyl ester, Fluorogenic esterase substrate, 50MG
MW 626.35 g/mol, Purity >96%. Cell-permeable, fluorogenic esterase substrate. Becomes hydrolyzed intracellularly to green fluorophore DCFA (ab145439) and covalently bonds to proteins.
The product is subject to the following: Abcam Restricted Use Statement
Abcam NBD-Cl, Amine-reactive fluorogenic dye, 1G
MW 199.55 Da, Purity >98%. Amine-reactive, fluorogenic dye. Forms green fluorescent adducts with aliphatic amines and thiols. Labels proteins, peptides, amino acids and other biomolecules.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Phosphatase Inhibitor Cocktail III, 1ML
Phosphatase Inhibitor Cocktail III (ab201114) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
The product is subject to the following: Abcam Restricted Use Statement
Vector Laboratories Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II) Biotinylated 2 mg
Griffonia simplicifolia lectin II (GSL II) is a dimeric glycoprotein composed of two subunits of nearly identical size. Each subunit has disulfide-linked chains and a binding site for α- or β-linkedN-acetylglucosamine residues. Unlike other N-acetylglucosamine-specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II is unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides. This conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Genscript Corporation Broad Multi Color Pre-Stained Protein Standard
Broad Multi Color Pre-Stained Protein Standard is a special-designed protein standard. The standard is a mixture of ten highly purified prestained proteins ranging from 5 kDa to 270 kDa, covalently coupled with four different chromophores. The 30 kDa band is red, 50 kDa band is green, 270 kDa band is orange, and other bands are blue. Broad Multi Color Pre-Stained Protein Standard is designed for observing protein separation during SDS-PAGE, verifying western transfer efficiency on membranes, and approximating the size of proteins.
Abcam Deubiquitinase Assay Kit
Deubiquitinase Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam Gamma H2A.X Staining Kit
Gamma H2A.X Staining Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam HomocitrullineCitrulline Assay Kit
HomocitrullineCitrulline Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam Alpha Galactosidase Activity Assay Kit
Alpha Galactosidase Activity Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam Gamma H2A.X Staining Kit
Gamma H2A.X Staining Kit
The product is subject to the following: Abcam Restricted Use Statement